-plot enzyme kinetic software version 14.0 Search Results


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ATCC anti west nile virus antibody responses 140
Anti West Nile Virus Antibody Responses 140, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Cusabio p selectin
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Elabscience Biotechnology p selectin elisa kit
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R&D Systems immuno adsorbent assay
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EUROIMMUN elisa
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R&D Systems human p selectin cd62p immunoassay
Human P Selectin Cd62p Immunoassay, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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CUSAg Inc rat elisa kit
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Jackson Immuno horseradish peroxidase hrp
Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by <t>horseradish</t> <t>peroxidase</t> <t>(HRP)-coupled</t> streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.
Horseradish Peroxidase Hrp, supplied by Jackson Immuno, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Bethyl chicken igm
Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by <t>horseradish</t> <t>peroxidase</t> <t>(HRP)-coupled</t> streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.
Chicken Igm, supplied by Bethyl, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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MyBiosource Biotechnology p3np elisa
Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by <t>horseradish</t> <t>peroxidase</t> <t>(HRP)-coupled</t> streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.
P3np Elisa, supplied by MyBiosource Biotechnology, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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SouthernBiotech human 140 igg h l
Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by <t>horseradish</t> <t>peroxidase</t> <t>(HRP)-coupled</t> streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.
Human 140 Igg H L, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by horseradish peroxidase (HRP)-coupled streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.

Journal: International Journal of Molecular Sciences

Article Title: Functions of Small Organic Compounds that Mimic the HNK-1 Glycan

doi: 10.3390/ijms21197018

Figure Lengend Snippet: Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by horseradish peroxidase (HRP)-coupled streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.

Article Snippet: The secondary donkey anti-mouse IgM antibody coupled to horseradish peroxidase (HRP) (cat #715-035-020) and secondary donkey anti-rabbit IgG antibody coupled to alexa fluor ® 488 (cat #711-545-152) were from Jackson ImmunoResearch (West Grove, PA, USA).

Techniques: Binding Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Inhibition