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Image Search Results
Journal: International Journal of Molecular Sciences
Article Title: Functions of Small Organic Compounds that Mimic the HNK-1 Glycan
doi: 10.3390/ijms21197018
Figure Lengend Snippet: Small organic compounds inhibit the binding of the HNK1-specific antibody to the HNK1 mimetic peptide in a concentration-dependent manner. ( a ) HNK1-specific antibody was substrate-coated on an enzyme-linked immunosorbent assay (ELISA) plate and preincubated with different concentrations (1–200 μM) of compounds from libraries or the negative control compound tacrine. Biotinylated HNK1 mimetic peptide was then added and detected by horseradish peroxidase (HRP)-coupled streptavidin followed by development with o-phenylenediamine dihydrochloride (OPD) substrate to produce a colored product that was quantified in the ELISA reader. The signal with this peptide without compounds was set to 100%. The graph shows average relative inhibition of the signal (duplicate wells carried out in three independent experiments ± SEM). All data points below the asterisks are different in inhibition when compared to the negative control compound tacrine (one-way ANOVA, F(31/64) = 4.122, p < 0.0001; Fisher’s PLSD test, * p < 0.05, ** p < 0.01). ( b ) Structure of the HNK1 mimetic compounds.
Article Snippet: The secondary donkey anti-mouse IgM antibody coupled to
Techniques: Binding Assay, Concentration Assay, Enzyme-linked Immunosorbent Assay, Negative Control, Inhibition